Mycology Lab ITS

Standard/reference strains for C. neoformans and C. gattii strain typing (1)

CBS #	ATCC#	FGS#	Other numbers	MAT & Serotype	Comments	Reference
*Cryptococcus neoformans*
*Cryptococcus neoformans var. grubii*
VNI (93, 96) = AFLP1 (6) = VN6 (VN5) (135)
CBS 10085	ATCC MYA-4564	10415	WM 148; W10; Brown	aA	1989, Australia, NSW, Sydney, clinical, CSF, HIV-, isolated by Sharon Chen	2, 3
CBS 8710	ATCC 48922	9487	DUMC 135.97; H99; NYSD 1649; CBS 10515; WM 04.15	aA	1978, USA, NC, Durham, clinical, CSF, patient with Hodgkin's lymphoma, isolated by John Perfect/Wiley Schell, type culture of C. neoformans var. grubii, genome sequence strain	4
VNII (93, 96) = AFLP1A (6) = VN7 (135)
CBS 10084	ATCC MYA-4565	10416	WM 626, W20; Cetin	aA	1993, Australia, NSW, Sydney, clinical, CSF, HIV-, isolated by Sharon Chen	2, 3
AD hybrid
VNIII (93, 96) = AFLP3 (6) = VN3 + VN4 (135)
CBS 10080	ATCC MYA-4566	10417	WM 628; 88B5400; Zapf	aA/aD	1988, Australia, VIC, Melbourne, clinical, CSF, HIV+, isolated by Bryan Speed	2, 3
CBS 132	ATCC 32045	-	CCRC 20528; DBVPG 6010; IFO 0608; IGC 3957; NRRL Y-2534	aA/aD	1894, Italy, environmental, fermenting fruit juice, isolated by F. Sanfelice, type culture for C. neoformans	5
Cryptococcus neoformans var. neoformans
VNIV (93, 96) = AFLP2 (6) = VN1 (VN2) (135)
CBS 10079	ATCC MYA-4567	10418	WM 629; B 87455, Borg, F 14	aD	1987, Australia, VIC, Melbourne, clinical, blood, HIV+, isolated by Bryan Speed	3
CBS 6900	ATCC 34873	10423	B-3501; DBVPG 6228; CBS 7697	aD	1975, USA, MD, Bethesda, NIH, crossing of NIH 12 x NIH 433, isolated by June Kwon-Chung	6
*Cryptococcus gattii*
VGI (96) = AFLP4 (6)
CBS 10078	ATCC MYA-4560	10419	WM 179; Bryon; H33.1; MH56	aB	1993, Australia, NSW, Sydney, clinical, CSF, HIV-, isolated by Sharon Chen	2, 7
CBS 6289	ATCC 32269	-	MUCL 30449, RV 20186; CBS 8273	aB	1966, Congo, Kinshasa, clinical, CSF, isolated by E. Gatti/R. Eeckels, type strain of C. neoformans var. gattii,	8
CBS 10510	-	-	WM 276; TCS ?SC1	aB	1993, Australia, NSW, Mt Annan National Park, environmental, Eycalyptus tereticornis woody debris, isolated by Tania Sorrell/Sharon Chen, genome sequence strain	7
VGII (96) = AFLP6 (6)
CBS 10082	ATCC MYA-4561	10420	WM 178; 49435; Colter; IFM 50894	aB	1991, Australia, NSW, Sydney, clinical, CSF, HIV-, isolated by Sharon Chen	7
CBS 10514	-	-	CDC R265; WM 02.32	aB	2001, Canada, BC, Duncan, Vancouver Island, clinical, bronchial wash, isolated by British Columbia CDC, high virulent Vancouver Island outbreak strain, VGIIa, genome sequence strain	9
VGIII (96) = AFLP5 (6)
CBS 10081	ATCC MYA-4562	10421	WM 175; WM 161; E698; 689; TP 0689; D1.13H	aB	1992, USA, California, San Diego, Blind Recreation Center / Park Boulevard UPAS street, environmental, Eucalyptus spp. woody debris, isolated by Tania Pfeifer/David Ellis	7, 10
CBS 6955	ATCC 32608	10424	DBVPG 6225; MUCL 30454; NIH 191; CBS 6916	aC	Before 1970, USA, San Fernando, California, clinical, CSF.	11
VGIV (96) = AFLP7 (11)
CBS 10101	ATCC MYA-4563	10422	WM 779; King Cheetah; IFM 50896	aC	1994, South Africa, Johannesburg, veterinary, Cheetah, isolated by Valarie Davis	12, 7

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5. Sanfelice, F. 1894. Contributo alla morfologia e biologia dei blastomiceti che si sviluppano nei succhi di alcuni frutti. Ann. Igien. 463?495.

6. Varma, A., and K. J. Kwon-Chung. 1989. Restriction fragment polymorphism in mitochondrial DNA of Cryptococcus neoformans. J Gen Microbiol 135:3353-3362.

7. W. Meyer, A. Castaneda, S. Jackson, M. Huynh, and E. Castaneda. 2003. Molecular typing of IberoAmerican Cryptococcus neoformans isolates. EID 9:189-195.

8. F. Gatti, and H. Eeckels. 1970. An atypical strain of Cryptococcus neoformans (Sanfelice) Vuillemin. Part I Description of the diseases and of the strain. Ann Soc Belge M�d Trop 50:689-694.

9. Kidd, S. E., F. Hagen, R. L. Tscharke, M. Huynh, K. H. Bartlett, M. Fyfe, L. MacDougall, T. Boekhout, K. J. Kwon-Chung, W. Meyer. 2004. A rare genotype of Cryptococcus gattii caused the cryptococcosis outbreak on Vancouver Island (British Columbia, Canada). Proc Natl Acad Sci U S A 101:17258-17263.

10. Sorrell, T. C., A. G. Brownlee, P. Ruma, R. Malik, T. J. Pfeiffer, and D. H. Ellis 1996b. Natural environmental sources of Cryptococcus neoformans var. gattii. J Clin Microbiol 34:1261-1263.

11. Kwon-Chung, K. J., J. E. Bennett, and T. S. Theodore. 1978. Cryptococcus bacillisporus sp. nov. serotype B-C of Cryptococcus neoformans, Int J Syst Bacteriol 28:616-620.

12. Bolton, L.A., R. G. Lobetti, D. N. Evezard, J. A. Picard, J. W. Nesbit, J. van Heerden, and R. E. Burroughs. 1990. Cryptococcosis in captive cheetah (Acinonyx jubatus): two cases. J S Afr Vet Assoc 70:35?39.